REVELATION OF MECHANISM OF ACTION OF RHIZOPHORA MUCRONATA POIR. BARK EXTRACT FOR ITS ANTIDIABETIC ACTIVITY BY GUT PERFUSION AND SIX SEGMENT METHOD IN LONG EVANS RATS

The aqueous bark extract of Rhizophora mucronata Poir. is used in Bangladesh as a hypoglycemic aid without any knowledge about its probable mode of action . It is an effort to assess the claimed hypoglycemic property of the crude drug and to get some knowledge about it. The Hypoglycemic effects were investigated in the ethanol extract of bark of Rhizophora mucronata Poir on Long Evans rats. Gut perfusion and six segments studies were carried out to assess these activities. In gut-perfusion study the percentage of glucose absorption in control rats vs. rats fed with 500 mg/kg extracts were observed at 5, 10, 15, 20, 25 and 30 minutes and the significant (p<0.05) absorption result was found which were respectively 30.71 vs. 56.34, 36.87 vs. 71.30, 35.87 vs. 62.11, 36.64 vs. 70.44, 36.36 vs. 64.21, 35.24 vs. 56.32, The percentage drug unabsorbed in GIT was better with 500 mg/kg than 250 mg/kg. The six-segment study was performed to assess the amount of sucrose remaining in the GIT at six different positions. The amount of sucrose unabsorbed in different GIT segments showed that in control rats vs. rats fed with 500mg/kg extract at 30 minutes in mmol/l was 0.1526 vs. 0.1767 which gradually abating with time dependent manner at 60, 180, and 360 minutes in mmol/l. These results suggests Rhizophora mucronata bark has significant dose dependant anti-diabetic effects, which significantly suppressed postprandial hyperglycemia after sucrose ingestion and reversibly increases the unabsorbed sucrose content throughout the gut