Open AccessSIMULTANEOUS SPECTROPHOTOMETRIC DETERMINATION OF DIACEREIN AND ACECLOFENAC IN TABLETS BY CHEMOMETRIC METHODS
Author(s) -
Vijayageetha Ragupathy,
Shantha Arcot
Publication year - 2013
Publication title -
international research journal of pharmacy
Language(s) - English
Resource type - Journals
ISSN - 2230-8407
DOI - 10.7897/2230-8407.04345
Subject(s) - aceclofenac , partial least squares regression , chemometrics , absorbance , principal component regression , principal component analysis , chromatography , chemistry , correlation coefficient , mathematics , analytical chemistry (journal) , statistics
Simultaneous spectrophotometric determination of diacerein and aceclofenac was performed by partial least-squares (PLS) and principal component regression (PCR) methods do not require any priori graphical treatment of the overlapping spectra of two drugs in the mixture. The absorbance values in the UV-Vis spectra were measured for the 67 wavelength points (from 234-300) in the spectral region 200–400 nm considering the intervals of 1 nm. The calibration range was found to be 1-5 μg/ml for diacerein, 2-10 μg/ml for aceclofenac with a correlation coefficient of 0.9998(PLS), 0.9995(PCR) for diacerein and 0.9999 (PLS), 0.9997 (PCR) for aceclofenac. The validation of the multivariate methods was realized by analyzing the synthetic mixtures of diacerein and aceclofenac. The numerical calculations were performed with the ‘Unscrambler 10.1 X’ software. The chemometrics analysis methods were satisfactorily applied to the simultaneous determination of diacerein and aceclofenac in the pharmaceutical formulation