Open AccessSYNTHESIS OF SILVER NANOPARTICLES BY CHEMICAL REDUCTION METHOD AND THEIR ANTIFUNGAL ACTIVITY
Author(s) -
Aashritha Shenava
Publication year - 2013
Publication title -
international research journal of pharmacy
Language(s) - English
Resource type - Journals
ISSN - 2230-8407
DOI - 10.7897/2230-8407.041024
Subject(s) - antifungal , silver nanoparticle , chemistry , reduction (mathematics) , nanoparticle , combinatorial chemistry , nanotechnology , microbiology and biotechnology , biology , materials science , mathematics , geometry
The aim of this study was to evaluate the antimicrobial activity of silvercolloidal nanoparticles which were synthesised by chemical reduction. Silver nanoparticles were synthesized by reduction of silver nitrate with sodium citrate. The presence of silver nanoparticles was detected by atomic absorption spectroscopy. Antifungal activity of silver nanoparticles was detected by the zone of inhibition. Silver nanoparticles exhibited a characteristic surface plasmon resonance band that is measured by UV-Vis spectroscopy, showing a typical absorbance peak for nanoparticles centred at 430 nm. The antifungal activity of silver nanoparticles was measured by the zones of inhibition by Kirby Bauer sensitivity testing which were measured after 24 h of incubation at 370C of Candida albicans growth on sabouraud dextrose agar. This study, integrates nanotechnology leading to possible advances in the formulation of new types of fungicide