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Evaluation of Left Ventricular Regional Systolic Function Using Tissue Doppler Echocardiography After Mesenchymal Stem Cell Transplantation in Rabbits With Myocardial Infarction
Author(s) -
Jiang Shuangquan,
Li Hairu,
Ren Min,
Tian Jiawei,
Su Yanxin,
Leng Xiaoping
Publication year - 2015
Publication title -
journal of ultrasound in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 91
eISSN - 1550-9613
pISSN - 0278-4297
DOI - 10.7863/ultra.34.7.1217
Subject(s) - medicine , transplantation , ligation , cardiology , myocardial infarction , mesenchymal stem cell , infarction , saline , pathology
Objectives The aim of this work was to assess left ventricular (LV) regional systolic function in rabbits with myocardial infarction after allogeneic mesenchymal stem cell (MSC) transplantation using quantitative tissue velocity imaging. Methods Thirty New Zealand White rabbits were assigned into 3 groups randomly: a sham‐operated group (n = 10), a myocardial infarction (MI) group (n = 10), and a MSC transplantation group (n = 10). Mesenchymal stem cells (1 × 10 7 in total) were delivered into 5 spots around the left anterior descending artery (LAD) blood supply area via direct intramyocardial injections 1 hour after LAD ligation in the MSC group, whereas the MI group received the same amount of phosphate‐buffered saline injections. Echocardiography was performed before LAD ligation and 1 day and 2 weeks after MSC transplantation, respectively. The peak systolic velocity (V s ) of each LV wall segment was measured. The myocardial slices were harvested for histologic staining after the last echocardiographic examination. Results The velocity curves for the LV myocardium before LAD ligation had a trend showing that the V s value decreased gradually from basal to apical segments. The V s values for the LV segments around the infarcted area in the MSC group decreased significantly compared with the sham group ( P < .05) 1 day after MSC transplantation, whereas they increased significantly 2 weeks after MSC transplantation compared with 1 day after LAD ligation ( P < .05). Conclusions This study demonstrates that quantitative tissue velocity imaging may provide a promising approach to quantitatively assessing LV regional systolic function before and after MSC transplantation.