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Ultrasonically induced in vitro cell lysis: node‐antinode interactions
Author(s) -
Doida Y,
Brayman A A,
Miller M W
Publication year - 1992
Publication title -
journal of ultrasound in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 91
eISSN - 1550-9613
pISSN - 0278-4297
DOI - 10.7863/jum.1992.11.8.413
Subject(s) - lysis , medicine , cell , in vitro , suspension (topology) , tube (container) , biophysics , bubble , microbiology and biotechnology , mechanics , immunology , biochemistry , biology , composite material , materials science , physics , mathematics , homotopy , pure mathematics
An attempt was made to discriminate between two hypotheses (standing wave, bubble recycling) of the mechanism of ultrasonically induced cell lysis in a rotating tube. A tube containing an aqueous suspension of P‐388 cells was moved back and forth (+/‐ 3 or +/‐ 7 mm) during insonation (1 MHz, 5 W/cm2, continuous wave, 5 min). Cell lysis (approximately 20%) occurred. As a positive control, some tubes were also partially or completely rotated during insonation; considerable cell lysis (approximately 60%) occurred. The results are interpreted to suggest that both hypotheses are simultaneously useful in explaining the observed effect of cell lysis in a rotating tube.