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Method of cavitation‐suppressed exposure of cells and explant mouse embryos to clinical real‐time and pulsed Doppler ultrasound.
Author(s) -
Madsen E L,
Frank G R,
Macdonald M C,
Martin A O,
Bouck N P,
Iannaccone P M
Publication year - 1991
Publication title -
journal of ultrasound in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 91
eISSN - 1550-9613
pISSN - 0278-4297
DOI - 10.7863/jum.1991.10.11.629
Subject(s) - ultrasound , medicine , explant culture , biomedical engineering , embryo , cavitation , suspension (topology) , anatomy , pathology , microbiology and biotechnology , acoustics , radiology , in vitro , biology , biochemistry , physics , mathematics , homotopy , pure mathematics
An apparatus and procedure for well‐controlled exposure of cells or explant mouse embryos to clinical real‐time ultrasound are described. Cells or embryos to be exposed are suspended in media made sufficiently viscous through inclusion of methylcellulose that cavitation is suppressed but thermal effects remain negligible. During exposure, the scanning beam is precisely centered in a 2 mm x 20 mm slot in a 20 cm diameter agar disc containing the suspension. The high viscosity causes the cells to remain distributed uniformly throughout the exposure; this fact, along with precision beam alignment, ensures that exposure is well defined. Exposure data are acquired with a 0.6 mm diameter hydrophone.