Premium
Ultrasonically activated gas‐filled micropores release hemoglobin and antigens from human erythrocytes in vitro.
Author(s) -
Miller D L,
Lamore B J
Publication year - 1987
Publication title -
journal of ultrasound in medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 91
eISSN - 1550-9613
pISSN - 0278-4297
DOI - 10.7863/jum.1987.6.5.231
Subject(s) - antigen , hemoglobin , lysis , hemolysis , in vitro , medicine , chromatography , membrane , red blood cell , biophysics , microbiology and biotechnology , immunology , chemistry , biochemistry , biology
Antigen release from human type B red blood cells was investigated by sensitive capillary‐tube assay techniques after subjecting 3% suspensions to a controlled form of ultrasonic cavitation. Cells were kept suspended during the 1,000‐sec exposures by an orbital motion of the sample chamber. Several spatial peak intensities of continuous 1.7 MHz ultrasound were applied to the samples which included gas‐filled 4‐microns diameter micropores in hydrophobic membranes. Free hemoglobin, indicating hemolysis, and free antigenic material, indicating solubilization of antigens, were found in the supernates of suspensions exposed to 90 mW/cm2 or 180 mW/cm2, respectively, or greater intensities. None of the free antigenic material could be attributed to antigen loss by surviving cells. For these conditions, the antigen release effect of ultrasound appears to be a byproduct of cavitation‐induced cell lysis.