Open AccessIdentification of Streptococcus pyogenes – Phenotypic Tests vs Molecular Assay ( spy 1258PCR): A Comparative Study
Author(s) -
Tintu Abraham,
Sujatha Sistla
Publication year - 2016
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2016/20053.8093
Subject(s) - bacitracin , latex fixation test , streptococcus pyogenes , microbiology and biotechnology , biology , genotype , polymerase chain reaction , genetics , antibiotics , antibody , gene , bacteria , staphylococcus aureus
Traditionally Group A Streptococcus pyogenes (GAS) is differentiated from other beta haemolytic streptococci (BHS) by certain presumptive tests such as bacitracin sensitivity and production of Pyrollidonyl Aryl Sulfatase (PYR). The phenotypic and genotypic confirmatory tests are Lancefield grouping for cell wall carbohydrate antigen and PCR for spy1258 gene respectively. Reliance on presumptive tests alone may lead to misidentification of isolates.