Open AccessBuccal Micronucleus Cytome Assay in Sickle Cell Disease
Author(s) -
Mallika Bokka Sri Satya Naga
Publication year - 2016
Publication title -
journal of clinical and diagnostic research
Language(s) - Uncategorized
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2016/19984.7998
Subject(s) - micronucleus , micronucleus test , buccal swab , buccal administration , biology , genome instability , genetics , somatic cell , pathology , colchicine , immunology , andrology , dna damage , medicine , gene , dna , bioinformatics , toxicity
Sickle Cell Anaemia (SCA) is a commonly inherited blood disorder preceded by episodes of pain, chronic haemolytic anaemia and severe infections. The underlying phenomenon which causes this disease is the point mutation in the haemoglobin beta gene (Hbβ) found on chromosome 11 p. Increased oxidative stress leads to DNA damage. DNA damage occurring in such conditions can be studied by the buccal micronucleus cytome assay, which is a minimally invasive method for studying chromosomal instability, cell death and regenerative potential of human buccal tissue.