Open AccessIdentification of HIV Mutation as Diagnostic Biomarker through Next Generation Sequencing
Author(s) -
Wen Hui Shaw,
Qianqian Lin,
Zikry Zhiwei Bin Roslee Muhammad,
Jia Jun Lee,
Wei Xin Khong,
Oon Tek Ng,
Eng Lee Tan,
Peng Li
Publication year - 2016
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2016/19760.8140
Subject(s) - virology , biology , genotyping , sanger sequencing , polymerase chain reaction , dna sequencing , reverse transcriptase , genome , gene , genetics , genotype
Current clinical detection of Human immunodeficiency virus 1 (HIV-1) is used to target viral genes and proteins. However, the immunoassay, such as viral culture or Polymerase Chain Reaction (PCR), lacks accuracy in the diagnosis, as these conventional assays rely on the stable genome and HIV-1 is a highly-mutated virus. Next generation sequencing (NGS) promises to be transformative for the practice of infectious disease, and the rapidly reducing cost and processing time mean that this will become a feasible technology in diagnostic and research laboratories in the near future. The technology offers the superior sensitivity to detect the pathogenic viruses, including unknown and unexpected strains.