Open AccessQuantification of DNA Extracted from Formalin Fixed Paraffin-Embeded Tissue Comparison of Three Techniques: Effect on PCR Efficiency
Author(s) -
Dushyant Kumar,
Manoj Kumar Panigrahi,
Moushumi Suryavanshi,
Anurag Mehta,
Kandarpa Kumar Saikia
Publication year - 2016
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2016/19383.8407
Subject(s) - fluorometer , dna , microbiology and biotechnology , primer dimer , polymerase chain reaction , real time polymerase chain reaction , digital polymerase chain reaction , biology , chemistry , fluorescence , gene , genetics , multiplex polymerase chain reaction , physics , quantum mechanics
Mutation detection from Formalin Fixed Paraffin-Embedding (FFPE) tissue in molecular lab became a necessary tool for defining potential targeted drug. Accurate quantification of DNA extracted from FFPE tissue is necessary for downstream applications like Polymerase Chain Reaction (PCR), sequencing etc.