Open AccessGlobal DNA Methylation Level among Ciprofloxacin-Resistant Clinical Isolates of Escherichia coli
Author(s) -
Thiyagarajan Yugendran,
Belgode Narasimha Harish
Publication year - 2016
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2016/19034.7830
Subject(s) - dna methylation , genomic dna , methylation , biology , plasmid , ciprofloxacin , escherichia coli , microbiology and biotechnology , dna , gene , genetics , bacteria , gene expression
Fluoroquinolone resistant clinical isolates belonging to the family Enterobacteriaceae is a major public health concern in India. Data analysis in JIPMER hospital revealed 10% rise in fluoroquinolone resistance within a span of three years suggestive of the possible involvement of mechanism/s other than QRDR capable of imparting fluoroquinolone resistance. DNA methylation regulates gene expression. Moreover, methylated cytosine is a mutational hotspot. Thus, DNA methylation can alter bacterial gene expression profile as well as facilitate the bacteria in accumulating mutations possibly leading to increased antimicrobial resistance. Therefore, the present study was carried out to identify the potential involvement of DNA methylation in ciprofloxacin resistance.