Open AccessCorrelation of RAPD-PCR Profiles with ESBL Production in Clinical Isolates of Klebsiella pneumoniae in Tehran
Author(s) -
Fereshteh Eftekhar,
Parvaneh K. Nouri
Publication year - 2015
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2015/10651.5373
Subject(s) - rapd , microbiology and biotechnology , klebsiella pneumoniae , cefepime , biology , amikacin , imipenem , aztreonam , piperacillin , ceftazidime , nalidixic acid , cefotaxime , ciprofloxacin , antibiotic resistance , antibiotics , medicine , genetic diversity , genetics , bacteria , population , escherichia coli , gene , environmental health , pseudomonas aeruginosa
Multidrug resistant K. pneumoniae, particularly the extended-spectrum β-lactamase (ESBL) producing strains, are often responsible for the failure of antibiotic treatment in nosocomial infections. Employing molecular methods to distinguish between ESBL and non-ESBL producing isolates can help quick identification of these multidrug resistant pathogens and thereby initiating appropriate antibiotic therapy. The aim of this study was to employ RAPD-PCR to distinguish the genetic fingerprints of ESBL producing clinical isolates of K. pneumoniae from ESBL negative strains.