Open AccessFrequency of Sperm DNA Fragmentation According to Selection Method: Comparison and Relevance of a Microfluidic Device and a Swim-up Procedure
Author(s) -
Kanako Kishi
Publication year - 2015
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2015/10332.6811
Subject(s) - sperm , asthenozoospermia , semen , microfluidics , dna fragmentation , centrifugation , andrology , biology , chromatography , biomedical engineering , chemistry , materials science , genetics , nanotechnology , male infertility , medicine , infertility , pregnancy , apoptosis , programmed cell death
Multiple rounds of centrifugation or washing spermatozoa can cause sperm DNA fragmentation (SDF); however, a microfluidic approach to select spermatozoa does not require centrifugation. Reports have suggested that sperm sorting using a microfluidic device is an effective method to select good quality spermatozoa, however, it is not known whether it reduces sperm DNA damage. We investigated whether the frequency of SDF was affected by selection method during sperm processing.