Open AccessEvaluation of Cytotoxicity of Silorane and Methacrylate based Dental Composites using Human Gingival Fibroblasts
Author(s) -
Prashanthi S Madhyastha,
Dilip G Naik,
Ravindra Kotian,
Divya Padma,
Srikant Natarajan,
Kumar Mr Bhat
Publication year - 2015
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2015/10269.5366
Subject(s) - cytotoxicity , mtt assay , toxicity , viability assay , fibroblast , chemistry , cell growth , methacrylate , in vitro , microbiology and biotechnology , dentistry , medicine , biochemistry , biology , monomer , polymer , organic chemistry
The effects of leached substances from the restorative dental materials may induce local and systemic adverse effects. Thus the biological and toxic properties of the restorative dental materials must be compatible with the oral tissues or with general health. Therefore, the need for biocompatible restorative dental material implies the necessity of toxicity testing. It was the purpose of this investigation to determine and compare the possible toxic effect of silorane based composite (Filtek P90) on human gingival fibroblast (HGF) in vitro using cytotoxicity measuring parameters (MTT assay) in comparison with its methacrylate counterpart (Z100) for their viability, proliferation rate.