Open AccessRole of S-100 Immunostaining in Demonstration of Nerve Changes and Quantification of Dendritic Cells in Leprosy
Author(s) -
Anand Mohanraj,
Sowmya Subramanian
Publication year - 2014
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2014/6436.4100
Subject(s) - leprosy , h&e stain , immunostaining , pathology , stain , medicine , tuberculoid leprosy , staining , nerve biopsy , immunoperoxidase , eosin , histopathology , lepromatous leprosy , immunohistochemistry , immunology , antibody , monoclonal antibody , peripheral neuropathy , endocrinology , diabetes mellitus
A definitive diagnosis of leprosy is based on a demonstration of either acid-fast bacilli or nerve elements within the granulomas. On routine hematoxylin and eosin stains, the nerve fibers are not easily identifiable. In this study, S-100 immunostain is used to highlight the nerve elements and to demonstrate and compare the nerve changes in spectrum of leprosy including reactions.