Open AccessEvaluation of Nested Polymerase Chain Reaction for Rapid Diagnosis of Clinically Suspected Tuberculous Pleurisy
Author(s) -
Manmeet Kaur Gill,
Sahiba Kukreja,
Namrata Chhabra
Publication year - 2013
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2013/6255.3577
Subject(s) - nested polymerase chain reaction , polymerase chain reaction , tuberculosis , gold standard (test) , mycobacterium tuberculosis , medicine , adenosine deaminase , mycobacterium tuberculosis complex , pleural fluid , microbiology and biotechnology , pathology , virology , biology , pleural effusion , gene , adenosine , biochemistry
Early diagnosis of tuberculosis is important in its control. The conventional techniques like smear microscopy and culture suffer from low sensitivity for diagnosis of extra-pulmonary tuberculosis like Pleural Tuberculosis (PTB) due to paucibacillary nature of the fluid. Polymerase Chain Reaction (PCR) is presently seen as a promising alternative to conventional techniques. In this study we have evaluated IS6110 sequence based nested PCR (nPCR) for the detection of Mycobacterium tuberculosis (MTB) DNA directly from clinical samples. The results of PCR were compared with the results of conventional methods like smear, culture and Adenosine Deaminase (ADA) activity.