Open AccessModified Double Disc Synergy Test to Detect ESBL Production in Urinary Isolates of Escherichia coli and Klebsiella pneumoniae
Author(s) -
Jaspal Kaur,
Shashi Chopra,
Sheevani,
Gomty Mahajan
Publication year - 2013
Publication title -
journal of clinical and diagnostic research
Language(s) - English
Resource type - Journals
eISSN - 2249-782X
pISSN - 0973-709X
DOI - 10.7860/jcdr/2013/4619.2734
Subject(s) - cefepime , cephalosporin , cefotaxime , klebsiella pneumoniae , microbiology and biotechnology , amoxicillin , ceftriaxone , escherichia coli , biology , antibiotics , antibiotic resistance , biochemistry , imipenem , gene
Various phenotypic methods are recommended in the routine practice to detect the ESBL production in gram negative bacilli. Among them, the Double Disc Synergy Test (DDST) which uses the third generation cephalosporins (3GC), is a simple and a reliable method. But the coexistence of AmpC may give false negative results. In such cases, the ESBL detection can be improved by using cefepime along with the third generation cephalosporins in DDST.