Open AccessHacia el esclarecimiento del rol del anión cloruro en la hipertensión arterial: su vínculo con el daño oxidativo en el riñón
Author(s) -
Nicolás Martín Kouyoumdzian,
Gabriel Kim,
Gabriel D. Robbesaul,
Paula D. Prince,
Ana M. Puyó,
Belisario E. Fernández,
Mónica Galleano,
Marcelo Roberto Choi
Publication year - 2021
Publication title -
revista argentina de cardiologia
Language(s) - English
DOI - 10.7775/rac.es.v89.i2.20034
Subject(s) - tbars , glutathione peroxidase , chemistry , sodium , endocrinology , medicine , oxidative stress , lipid peroxidation , renal function , renal cortex , superoxide dismutase , blood pressure , kidney , biochemistry , organic chemistry
The role of the chloride anion on the deleterious effects of excessive consumption of salt (NaCl) and whether its effects are independent each other of the presence of sodium remains to date, unknown and unclear. Objective: To demonstrate that both a chloride overload and a sodium overload in the diet produce deleterious effects, by different mechanisms, on systolic blood pressure (SBP), renal function and markers of oxidative stress in the kidney. Materials and Methods: Male Wistar rats were divided into four groups (n = 8 / group) and fed with different diets for three weeks: C: control (standard diet), and diets: NaCl: hypersodic-hyperchloric; Na: hypersodic without chloride and Cl: hyperchloric without sodium. Systolic blood pressure (SBP) and renal function were determined, and the production of thiobarbituric acid reactive species (TBARS) and the activity and expression of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) enzymes were evaluated in renal cortex tissue. Results: SBP increased (*) in the two groups fed with chloride. The fractional excretion of sodium and chloride increased (*) in the NaCl and Na groups. increased (*) in the renal cortex with the three diets. No changes were observed in the activity and expression of SOD and CAT. GPx activity increased (*) in the two groups that received chloride; (* p <0.05 vs C). Conclusion: Both sodium and chloride overload are associated with a higher oxidative state characterized by an increase in lipid peroxidation in the renal cortex. However, compared with Na group, only chloride overload is associated with higher GPx activity and hypertension without any changes in urinary chloride excretion, suggesting a higher renal pro-oxidant state in this experimental group.