Open AccessTransient expression of fusion and hemagglutinin-neuraminidase epitopes of Newcastle disease virus in maize as a potent candidate vaccine
Author(s) -
Amir Ghaffar Shahriari,
Ali Niazi,
Maziar Habibi-Pirkoohi
Publication year - 2021
Publication title -
clinical and experimental vaccine research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.958
H-Index - 11
eISSN - 2287-366X
pISSN - 2287-3651
DOI - 10.7774/cevr.2021.10.3.245
Subject(s) - agroinfiltration , biology , nicotiana benthamiana , virology , expression vector , epitope , fusion protein , hemagglutinin (influenza) , agrobacterium , virus , gene , transgene , microbiology and biotechnology , recombinant dna , genetics , antibody
Newcastle disease (ND) represents a major viral disease across the world which imposes high costs to poultry producers for vaccination. Hemagglutinin-neuraminidase (HN) and fusion (F) proteins are the major immunogenic epitopes of Newcastle disease virus and hence, have been the main targets for development of anti-ND vaccines. This paper reports transient expression of a synthetic gene composing of four tandem repeats of HN and three tandem repeats of F epitopes in maize leaves as initial step toward production of recombinant vaccine against ND.