Open AccessApplication of carbon dioxide to the skin and muscle oxygenation of human lower-limb muscle sites during cold water immersion
Author(s) -
Mitsuo Yoshimura,
Tatsuya Hojo,
Hidehiro Yamamoto,
Misato Tachibana,
Masatoshi Nakamura,
Hiroaki Tsutsumi,
Yoshiyuki Fukuoka
Publication year - 2020
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.9785
Subject(s) - oxygenation , carbon dioxide , chemistry , vasodilation , vasoconstriction , anatomy , blood flow , isometric exercise , deoxygenation , oxygen , anesthesia , medicine , zoology , biology , biochemistry , organic chemistry , catalysis
Background Cold therapy has the disadvantage of inducing vasoconstriction in arterial and venous capillaries. The effects of carbon dioxide (CO 2 ) hot water depend mainly on not only cutaneous vasodilation but also muscle vasodilation. We examined the effects of artificial CO 2 cold water immersion (CCWI) on skin oxygenation and muscle oxygenation and the immersed skin temperature. Subjects and Methods Fifteen healthy young males participated. CO 2 -rich water containing CO 2 >1,150 ppm was prepared using a micro-bubble device. Each subject’s single leg was immersed up to the knee in the CO 2 -rich water (20 °C) for 15 min, followed by a 20-min recovery period. As a control study, a leg of the subject was immersed in cold tap-water at 20 °C (CWI). The skin temperature at the lower leg under water immersion (T sk -WI) and the subject’s thermal sensation at the immersed and non-immersed lower legs were measured throughout the experiment. We simultaneously measured the relative changes of local muscle oxygenation/deoxygenation compared to the basal values (Δoxy[Hb+Mb], Δdeoxy[Hb+Mb], and Δtotal[Hb+Mb]) at rest, which reflected the blood flow in the muscle, and we measured the tissue O 2 saturation (S t O 2 ) by near-infrared spectroscopy on two regions of the tibialis anterior (TA) and gastrocnemius (GAS) muscles. Results Compared to the CWI results, the Δoxy[Hb+Mb] and Δtotal[Hb+Mb] in the TA muscle at CCWI were increased and continued at a steady state during the recovery period. In GAS muscle, the Δtotal[Hb+Mb] and Δdeoxy[Hb+Mb] were increased during CCWI compared to CWI. Notably, S t O 2 values in both TA and GAS muscles were significantly increased during CCWI compared to CWI. In addition, compared to the CWI, a significant decrease in T sk at the immersed leg after the CCWI was maintained until the end of the 20-min recovery, and the significant reduction continued. Discussion The combination of CO 2 and cold water can induce both more increased blood inflow into muscles and volume-related (total heme concentration) changes in deoxy[Hb+Mb] during the recovery period. The T sk -WI stayed lower with the CCWI compared to the CWI, as it is associated with vasodilation by CO 2 .