Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2 | Zendy

Yee Ling Lau | Zendy; Ilyiana Ismail | Zendy; Nur Izati Mustapa | Zendy; Meng Yee Lai | Zendy; Tuan Suhaila Tuan Soh | Zendy; Afifah Haji Hassan | Zendy; Kalaiarasu M. Peariasamy | Zendy; Yee Leng Lee | Zendy; Yoong Min Chong | Zendy; IChing Sam | Zendy; Pik Pin Goh | Zendy

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Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

Author(s) -

Yee Ling Lau,

Ilyiana Ismail,

Nur Izati Mustapa,

Meng Yee Lai,

Tuan Suhaila Tuan Soh,

Afifah Haji Hassan,

Kalaiarasu M. Peariasamy,

Yee Leng Lee,

Yoong Min Chong,

IChing Sam,

Pik Pin Goh

Publication year - 2020

Publication title -

peerj

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.927

H-Index - 70

ISSN - 2167-8359

DOI - 10.7717/peerj.9278

Subject(s) - loop mediated isothermal amplification , reverse transcription loop mediated isothermal amplification , reverse transcriptase , reverse transcription polymerase chain reaction , microbiology and biotechnology , nucleic acid , real time polymerase chain reaction , recombinase polymerase amplification , serial dilution , covid-19 , chemistry , polymerase chain reaction , biology , virology , medicine , dna , gene , messenger rna , biochemistry , alternative medicine , disease , pathology , infectious disease (medical specialty)

Background Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. Methods A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. Results This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.

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