Open AccessReal-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2
Author(s) -
Yee Ling Lau,
Ida Suriya Ismail,
Nur Izati Mustapa,
Meng Yee Lai,
Tuan Suhaila Tuan Soh,
Afifah Haji Hassan,
Kalaiarasu M Peariasamy,
Yee Leng Lee,
Yoong Min Chong,
IChing Sam,
Pik Pin Goh
Publication year - 2020
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.9278
Subject(s) - loop mediated isothermal amplification , reverse transcription loop mediated isothermal amplification , reverse transcriptase , reverse transcription polymerase chain reaction , microbiology and biotechnology , nucleic acid , real time polymerase chain reaction , recombinase polymerase amplification , serial dilution , covid-19 , chemistry , polymerase chain reaction , biology , virology , medicine , dna , gene , messenger rna , biochemistry , alternative medicine , disease , pathology , infectious disease (medical specialty)
Background Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. Methods A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. Results This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.