Open AccessBioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia
Author(s) -
Rhesi Kristiana,
Gilles Bedoux,
Gerard Pals,
I Wayan Mudianta,
Laure Taupin,
Christel Marty,
Meezan Ardhanu Asagabaldan,
Diah Ayuningrum,
Agus Trianto,
Nathalie Bourgoug,
Ocky Karna Radjasa,
Agus Sabdono,
Muhammad Hanafi
Publication year - 2020
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.8093
Subject(s) - pseudoalteromonas , microbiology and biotechnology , bacteria , cytotoxicity , vero cell , staphylococcus aureus , biology , marine bacteriophage , cytotoxic t cell , virus , in vitro , biochemistry , virology , 16s ribosomal rna , genetics
The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500–1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra , whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius . The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5–1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius , had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant.