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Background   Hibiscus hamabo  Sieb. et Zucc. is a semi-mangrove plant used for the ecological restoration of saline-alkali land, coastal afforestation and urban landscaping. The genetic transformation  H. hamabo  is currently inefficient and laborious, restricting gene functional studies on this species. In plants, virus-induced gene silencing provides a pathway to rapidly and effectively create targeted gene knockouts for gene functional studies.    Methods  In this study, we tested the efficiency of a tobacco rattle virus vector in silencing the cloroplastos alterados 1 ( CLA1 ) gene through agroinfiltration.    Results  The leaves of  H. hamabo  showed white streaks typical of  CLA1  gene silencing three weeks after agroinfiltration. In agroinfiltrated  H. hamabo  plants, the  CLA1  expression levels in leaves with white streaks were all significantly lower than those in leaves from mock-infected and control plants.    Conclusions  The system presented here can efficiently silence genes in  H. hamabo  and may be a powerful tool for large-scale reverse-genetic analyses of gene functions in  H. hamabo .

Efficient virus-induced gene silencing in Hibiscus hamabo Sieb. et Zucc. using tobacco rattle virus