Open AccesslncRNA MALAT1 mediates osteogenic differentiation of bone mesenchymal stem cells by sponging miR-129-5p
Author(s) -
Jian Yin,
Zhanglong Zheng,
Xiaoli Zeng,
Yijie Zhao,
Zexin Ai,
Miao Yu,
Yang’ou Wu,
Jirui Jiang,
Jia Li,
Shengjiao Li
Publication year - 2022
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.13355
Subject(s) - malat1 , microrna , western blot , mesenchymal stem cell , microbiology and biotechnology , osteoblast , cellular differentiation , alkaline phosphatase , chemistry , runx2 , stem cell , competing endogenous rna , biology , long non coding rna , rna , gene , biochemistry , in vitro , enzyme
Background Bone mesenchymal stem cells (BMSCs) have good osteogenic differentiation potential and have become ideal seed cells in bone tissue engineering. However, the osteogenic differentiation ability of BMSCs gradually weakens with age, and the regulatory mechanism is unclear. Method We conducted a bioinformatics analysis, dual-luciferase reporter (DLR) experiment, and RNA binding protein immunoprecipitation (RIP) to explore the hub genes that may affect BMSC functions. Results The expression level of long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 ( Malat1 ) was significantly higher in the BMSCs from elderly than younger mice, while miR-129-5p showed the opposite trend. The results of alkaline phosphatase staining, quantitative reverse transcription PCR and western blot experiments indicated that inhibiting the expression of Malat1 inhibits the osteogenic differentiation of BMSCs. This effect can be reversed by reducing the expression of miR-129-5p . Additionally, DLR and RIP experiments confirmed that Malat1 acts as a sponge for miR-129-5p . Conclusion Overall, our study findings indicated that lncRNA Malat1 may play a critical role in maintaining the osteoblast differentiation potential of BMSCs by sponging miR-129-5p .