Open AccessZonula occludens-1 expression is reduced in nasal epithelial cells of allergic rhinitis patients
Author(s) -
Che Othman Siti Sarah,
Siti Muhamad Nur Husna,
Norasnieda Md Shukri,
Kah Keng Wong,
Noor Suryani Mohd Ashari
Publication year - 2022
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.13314
Subject(s) - histone deacetylase 2 , hdac1 , pathogenesis , messenger rna , immunology , tight junction , histone deacetylase , medicine , biology , andrology , chemistry , histone , gene , microbiology and biotechnology , genetics
Allergic rhinitis (AR) is a common allergic disease characterized by disruption of nasal epithelial barrier. In this study, we investigated the mRNA expression of zonula occludens-1 ( ZO-1 ), ZO-2 and ZO-3 and histone deacetylase 1 ( HDAC1 ) and HDAC2 in AR patients compared to healthy controls. RNA samples were extracted from nasal epithelial cells of house dust mites (HDMs)-sensitized AR patients and healthy controls ( n = 28 in each group). The RNAs were reverse transcribed into cDNAs for measurement of ZO-1 , ZO-2 , ZO-3 , HDAC1 and HDAC2 expression levels by quantitative PCR. The mRNA expression of ZO-1 was significantly decreased in AR patients compared to healthy controls ( p = 0.010). No significant difference was observed in the expression levels of ZO-2 , ZO-3 , HDAC1 and HDAC2 in AR patients compared to healthy controls. We found significant associations of higher HDAC2 levels in AR patients with lower frequency of changing bedsheet ( p = 0.043) and with AR patients sensitized to Dermatophagoides farinae ( p = 0.041). Higher expression of ZO-2 was observed in AR patients who had pets ( p = 0.007). In conclusion, our data indicated that ZO-1 expression was lower in AR patients contributing to decreased integrity of nasal epithelial barrier integrity, and HDAC2 may be involved in the pathogenesis of the disease.