Open AccessHydrogen sulphide alleviates Fusarium Head Blight in wheat seedlings
Author(s) -
Yuanyuan Yao,
Wenjie Kan,
Pengfei Su,
Yan Zhu,
Wei Zhong,
Jinfeng Xi,
Dacheng Wang,
Caiguo Tang,
Lifang Wu
Publication year - 2022
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.13078
Subject(s) - fusarium , malondialdehyde , coleoptile , hydrogen peroxide , germination , spore , reactive oxygen species , antioxidant , mycelium , mycotoxin , spore germination , inoculation , chemistry , horticulture , biology , food science , botany , biochemistry
Hydrogen sulphide (H 2 S), a crucial gas signal molecule, has been reported to be involved in various processes related to development and adversity responses in plants. However, the effects and regulatory mechanism of H 2 S in controlling Fusarium head blight (FHB) in wheat have not been clarified. In this study, we first reported that H 2 S released by low concentrations of sodium hydrosulphide (NaHS) could significantly alleviate the FHB symptoms caused by Fusarium graminearum ( F. graminearum ) in wheat. We also used coleoptile inoculation to investigate the related physiological and molecular mechanism. The results revealed that FHB resistance was strongly enhanced by the H 2 S released by NaHS, and 0.3 mM was confirmed as the optimal concentration. H 2 S treatment dramatically reduced the levels of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) while enhancing the activities of antioxidant enzymes. Meanwhile, the relative expressions levels of defence-related genes, including PR1.1 , PR2 , PR3 , and PR4 , were all dramatically upregulated. Our results also showed that H 2 S was toxic to F. graminearum by inhibiting mycelial growth and spore germination. Taken together, the findings demonstrated the potential value of H 2 S in mitigating the adverse effects induced by F. graminearum and advanced the current knowledge regarding the molecular mechanisms in wheat.