Open AccessA comparative study of bacterial diversity based on effects of three different shade shed types in the rhizosphere of Panax quiquefolium L.
Author(s) -
Xianchang Wang,
Xingzhong Guo,
Hou Li-juan,
Jiaohong Zhang,
Jing Hu,
Feng Zhang,
Ji-Lei Mao,
Zhifen Wang,
Congjing Zhang,
JinHee Han,
Yanbo Zhu,
Chao Liu,
Jinyue Sun,
Chenggang Shan
Publication year - 2022
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.12807
Subject(s) - rhizosphere , biology , ginseng , shading , botany , proteobacteria , sphingomonas , microorganism , 16s ribosomal rna , bacteria , medicine , art , genetics , alternative medicine , pathology , visual arts
Background Shading is an important factor affecting the cultivation of American ginseng, as it influences crop quality and yield. Rhizosphere microorganisms are also crucial for normal plant growth and development. However, whether different shade types significantly change American ginseng rhizosphere microorganisms is unknown. Methods This study evaluated the rhizosphere soils of American ginseng under traditional, high flag and high arch shade sheds. High-throughput 16S rRNA gene sequencing determined the change of rhizosphere bacterial communities. Results The microbial diversity in rhizosphere soils of American ginseng significantly changed under different shading conditions. The bacteria diversity was more abundant in the high arch shade than flat and traditional shades. Different bacterial genera, including Bradyrhizobium , Rhizobium , Sphingomonas , Streptomyces and Nitrospira , showed significantly different abundances. Different shading conditions changed the microbial metabolic function in the American ginseng rhizosphere soils. The three types of shade sheds had specific enriched functional groups. The abundance of ATP-binding cassette (ABC) transporters consistently increased in the bacterial microbiota. These results help understand the influence of shading systems on the rhizosphere microecology of American ginseng, and contribute to the American ginseng cultivation.