Open AccessInvestigation of lipolytic activity of the red king crab hepatopancreas homogenate by NMR spectroscopy
Author(s) -
Maria Timchenko,
Vladislav Molchanov,
Maxim Molchanov,
Alexander Timchenko,
Evgeny Sogorin
Publication year - 2022
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.12742
Subject(s) - hepatopancreas , monoacylglycerol lipase , balanus , biochemistry , chemistry , nuclear magnetic resonance spectroscopy , chromatography , hydrolysis , biology , crustacean , fishery , organic chemistry , endocannabinoid system , receptor
The digestive gland of craboids (hepatopancreas) is rich in a huge number of various enzymes (collagenases, nucleases, hyaluronidases, proteases), which are well studied at the moment. However, little is known about crustacean lipases. In this work, using 1 H NMR spectroscopy, it was found that the hepatopancreas homogenate of the red king crab Paralithodes camtschaticus demonstrates high lipolytic activity against triacetin in a wide pH range and shows moderate activity against the caprylic/capric triglyceride emulsion. Under the action of the hepatopancreas homogenate, triacylglycerols are converted into 1,2-diacylglycerol, and then into 2-monoacylglycerol and 1-monoacylglycerol. The 1-monoacylglycerol predominates in the reaction products. The use of NMR spectroscopy makes it possible to quickly detect hydrolysis products and evaluate the reaction direction.