Open AccessGenome-wide sequence identification and expression analysis of N6-methyladenosine demethylase in sugar beet (Beta vulgaris L.) under salt stress
Author(s) -
Jie Cui,
Junli Liu,
Junliang Li,
Dayou Cheng,
Chunhua Dai
Publication year - 2022
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.12719
Subject(s) - demethylase , biology , gene , gene family , genetics , abiotic stress , alkb , arabidopsis thaliana , phylogenetic tree , sugar beet , genome , protein family , arabidopsis , biochemistry , histone , mutant , horticulture , dna repair
In eukaryotes, N 6 -methyladenosine (m 6 A) is the most abundant and highly conserved RNA modification. In vivo , m 6 A demethylase dynamically regulates the m 6 A level by removing the m 6 A marker where it plays an important role in plant growth, development and response to abiotic stress. The confirmed m 6 A demethylases in Arabidopsis thaliana include ALKBH9B and ALKBH10B, both belonging to the ALKB family. In this study, BvALKB family members were identified in sugar beet genome-wide database, and their conserved domains, gene structures, chromosomal locations, phylogeny, conserved motifs and expression of BvALKB genes were analyzed. Almost all BvALKB proteins contained the conserved domain of 2OG-Fe II-Oxy. Phylogenetic analysis suggested that the ten proteins were clustered into five groups, each of which had similar motifs and gene structures. Three Arabidopsis m 6 A demethylase-homologous proteins (BvALKBH6B, BvALKBH8B and BvALKBH10B) were of particular interest in our study. Expression profile analysis showed that almost all genes were up-regulated or down-regulated to varying degrees under salt stress. More specifically, BvALKBH10B homologous to AtALKBH10B was significantly up-regulated, suggesting that the transcriptional activity of this gene is responsive to salt stress. This study provides a theoretical basis for further screening of m 6 A demethylase in sugar beet, and also lays a foundation for studying the role of ALKB family proteins in growth, development and response to salinity stress.