Open AccessDnajb8, a target gene of SOX30, is dispensable for male fertility in mice
Author(s) -
Fengsong Wang,
Shuai Kong,
Xuechun Hu,
Xin Li,
Bo Xu,
Qiuling Yue,
Kaiqiang Fu,
Lan Ye,
Shun Bai
Publication year - 2020
Publication title -
peerj
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.927
H-Index - 70
ISSN - 2167-8359
DOI - 10.7717/peerj.10582
Subject(s) - biology , meiosis , gene , mutant , phenotype , genetics , transcription factor , male fertility , spermiogenesis , transcription (linguistics) , crispr , mitosis , spermatogenesis , microbiology and biotechnology , sterility , fertility , population , sperm , linguistics , demography , philosophy , sociology , endocrinology
Background The DNAJ family of molecular chaperones maintains protein homeostasis in mitotic and postmeiotic cells, especially germ cells. Recently, we found that the transcription factor SOX30 initiates transcription of Dnajb8 during late meiosis and spermiogenesis in mouse testes. Methods We used the CRISPR/Cas9 system to generate Dnajb8 mutant mice and analyze the phenotype of the Dnajb8 mutants. Results Although Dnajb8 is an evolutionarily conserved gene, it is not essential for spermatogenesis and male fertility. We provide this phenotypic information, which could prevent duplicative work by other groups.