Clostridium manihotivorumsp. nov., a novel mesophilic anaerobic bacterium that produces cassava pulp-degrading enzymes | Zendy

Pattsarun Cheawchanlertfa | Zendy; Sawannee Sutheeworapong | Zendy; Piroon Jenjaroenpun | Zendy; Thidathip Wongsurawat | Zendy; Intawat Nookaew | Zendy; Supapon Cheevadhanarak | Zendy; Akihiko Kosugi | Zendy; Patthra Pason | Zendy; Rattiya Waeonukul | Zendy; Khanok Ratanakhanokchai | Zendy; Chakrit Tachaapaikoon | Zendy

Open Access

Clostridium manihotivorumsp. nov., a novel mesophilic anaerobic bacterium that produces cassava pulp-degrading enzymes

Author(s) -

Pattsarun Cheawchanlertfa,

Sawannee Sutheeworapong,

Piroon Jenjaroenpun,

Thidathip Wongsurawat,

Intawat Nookaew,

Supapon Cheevadhanarak,

Akihiko Kosugi,

Patthra Pason,

Rattiya Waeonukul,

Khanok Ratanakhanokchai,

Chakrit Tachaapaikoon

Publication year - 2020

Publication title -

peerj

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.927

H-Index - 70

ISSN - 2167-8359

DOI - 10.7717/peerj.10343

Subject(s) - biology , cellulase , genome , starch , bacteria , microbiology and biotechnology , gene , food science , cellulose , genetics , biochemistry

Background Cassava pulp is a promising starch-based biomasses, which consists of residual starch granules entrapped in plant cell wall containing non-starch polysaccharides, cellulose and hemicellulose. Strain CT4 T , a novel mesophilic anaerobic bacterium isolated from soil collected from a cassava pulp landfill, has a strong ability to degrade polysaccharides in cassava pulp. This study explored a rarely described species within the genus Clostridium that possessed a group of cassava pulp-degrading enzymes. Methods A novel mesophilic anaerobic bacterium, the strain CT4 T , was identified based on phylogenetic, genomic, phenotypic and chemotaxonomic analysis. The complete genome of the strain CT4 T was obtained following whole-genome sequencing, assembly and annotation using both Illumina and Oxford Nanopore Technology (ONT) platforms. Results Analysis based on the 16S rRNA gene sequence indicated that strain CT4 T is a species of genus Clostridium . Analysis of the whole-genome average amino acid identity (AAI) of strain CT4 T and the other 665 closely related species of the genus Clostridium revealed a separated strain CT4 T from the others. The results revealed that the genome consisted of a 6.3 Mb circular chromosome with 5,664 protein-coding sequences. Genome analysis result of strain CT4 T revealed that it contained a set of genes encoding amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes. A comparative genomic analysis of strain CT4 T with closely related species with available genomic information, C. amylolyticum SW408 T , showed that strain CT4 T contained more genes encoding cassava pulp-degrading enzymes, which comprised a complex mixture of amylolytic-, hemicellulolytic-, cellulolytic- and pectinolytic enzymes. This work presents the potential for saccharification of strain CT4 T in the utilization of cassava pulp. Based on phylogenetic, genomic, phenotypic and chemotaxonomic data, we propose a novel species for which the name Clostridium manihotivorum sp. nov. is suggested, with the type strain CT4 T (= TBRC 11758 T = NBRC 114534 T ).

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