Open AccessCloning and expression of a hypothetical Loosenin from Neurospora crassa
Author(s) -
Aranzazu Campos-Oliver,
Rosa Estela Quiroz-Castañeda,
Ernesto Ortiz-Suri,
Jorge Luis Folch-Mallol
Publication year - 2014
Publication title -
revista latinoamericana de biotecnología ambiental y algal
Language(s) - English
Resource type - Journals
ISSN - 2007-2570
DOI - 10.7603/s40682-014-0001-y
Subject(s) - neurospora crassa , cellulase , crassa , biochemistry , cloning (programming) , cellulose , biology , neurospora , gene , chemistry , computational biology , computer science , mutant , programming language
Loosenin is a protein recently described which presents amorphogenic activity on cellulose. It was isolated from the Basidiomycete Bjerkandera adusta and it enhances sugar release from cellulosic fibers treated previously with it and then subjected to cellulase treatment. It can also bind to other polysaccharides like chitin and xylans but presents no hydrolytic activity itself. Blast analysis using the loosenin amino acid sequence retrieved a sequence from the Neurospora crassa genome that showed 59% similarity. In this work, we cloned, expressed and partially characterized a putative loosenin from N. crassa, since these proteins have a potential for pretreatment of lignocellulosic materials.