Open AccessArchaeal chromatin ‘slinkies’ are inherently dynamic complexes with deflected DNA wrapping pathways
Author(s) -
Samuel Bowerman,
Jeff Wereszczynski,
Karolin Luger
Publication year - 2021
Publication title -
elife
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.879
H-Index - 139
ISSN - 2050-084X
DOI - 10.7554/elife.65587
Subject(s) - nucleosome , chromatin , histone , archaea , dna , biology , histone modifying enzymes , base pair , biophysics , microbiology and biotechnology , genetics , bacteria
Eukaryotes and many archaea package their DNA with histones. While the four eukaryotic histones wrap ~147 DNA base pairs into nucleosomes, archaeal histones form ‘nucleosome-like’ complexes that continuously wind between 60 and 500 base pairs of DNA (‘archaeasomes’), suggested by crystal contacts and analysis of cellular chromatin. Solution structures of large archaeasomes (>90 DNA base pairs) have never been directly observed. Here, we utilize molecular dynamics simulations, analytical ultracentrifugation, and cryoEM to structurally characterize the solution state of archaeasomes on longer DNA. Simulations reveal dynamics of increased accessibility without disruption of DNA-binding or tetramerization interfaces. Mg 2+ concentration influences compaction, and cryoEM densities illustrate that DNA is wrapped in consecutive substates arranged 90 o out-of-plane with one another. Without ATP-dependent remodelers, archaea may leverage these inherent dynamics to balance chromatin packing and accessibility.