Open AccessDNA-repair Deficient Strains Chlamydomonas reinhardtii as Good Tools for Environmental Toxicology
Author(s) -
Margarita Dimitrova,
Daniela A. Miteva,
Teodora Todorova,
Petya Parvanova,
Maria Yovkova,
Milen Dimitrov,
Stephka Chankova
Publication year - 2022
Publication title -
dokladi na bʺlgarskata akademiâ na naukite
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.244
H-Index - 17
eISSN - 2367-5535
pISSN - 1310-1331
DOI - 10.7546/crabs.2022.02.07
Subject(s) - chlamydomonas reinhardtii , genotoxicity , biology , dna repair , dna damage , microbiology and biotechnology , bioassay , dna , malondialdehyde , oxidative stress , genotype , genetics , gene , chemistry , biochemistry , toxicity , mutant , organic chemistry
The aim of the present study was to compare stress response of Chlamydomonas reinhardtii strains with different DNA repair capability to zeocin in order to develop a set of endpoints for the revealing of very low levels of xenobiotics. Four C. reinhardtii genotypes – 137C (wild type); CW15 (cell-wallless); UVS-10 (recombination-repair deficient); UVS-14 (mismatch-repair deficient) and different endpoints were applied – micro-colonies survival assay, malondialdehyde (MDA) and intracellular H2O2 quantities, carotenoids content, double-strand DNA breaks (DSBs) induction. Based on the cell survival, strains could be arranged as follows: WT > CW > UVS10 ∼ UVS14. The strongest oxidative stress, measured as the highest MDA and H2O2 contents, was evaluated for UVS-14 genotype. The highest quantity of DSBs induced was measured after zeocin treatment with low doses (2–10 μg/ml) for UVS-10 genotype (P < 0.01). Based on the data reported, a short-term bioassay based on C. reinhardtii with DNA repair-deficient genotypes is proposed to be successfully used in genotoxicity screening of low doses xenobiotics – micro-colonies assay on both strains (UVS-10 and UVS-14); measurement of the kinetics of MDA and H2O2 content on strain UVS-14, and primary induced DSBs levels on strain UVS-10.