Open AccessA nnovel wide field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination
Author(s) -
Wenchao Qiao,
Kaige Wang,
Yufeng Shao,
Junle Qu,
Hanben Niu
Publication year - 2013
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.62.034203
Subject(s) - optics , speckle pattern , materials science , microscope , microscopy , polarization (electrochemistry) , light sheet fluorescence microscopy , optical sectioning , image quality , wavelength , fluorescence , fluorescence microscope , optoelectronics , computer science , physics , artificial intelligence , chemistry , image (mathematics)
We propose a novel wide-field fluorescence sectioning microscope based on polarization filtering image enhancement and dynamic speckle illumination in this article. A new type of polarization filtering image enhancement technology, based on the differences in polarization characteristic between excitation light and fluorescence, is used to filter out the excitation light and enhance image quality. The dynamic speckle illumination is employed to achieve the wide field fluorescence sectioning which has many advantages, such as simple configuration, low cost, rapid response and easy operation. The experimental results indicate that our filtering method can be utilized to improve the image quality significantly, and the dynamic using speckle illumination can be employed to achieve optical sectioning with a wide field and a high vertical resolution capability. Our study is not only to enrich the technological method to extract the weak fluorescence signal from the strong excitation light, but also to provide a significant reference for developing a light wavelength tunable multi-spectral fluorescence microscope with rapid response.