Open AccessLipid exhange between membranes: effects of temperature and ionic strength
Author(s) -
鐘偉 江,
Guoliang Zhang,
Ji Ma,
Tao Zhu
Publication year - 2013
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.62.018701
Subject(s) - ionic strength , vesicle , quartz crystal microbalance , lipid bilayer , adsorption , materials science , membrane , biophysics , chemical engineering , bilayer , mechanical strength , ionic bonding , chemistry , chromatography , ion , composite material , organic chemistry , biochemistry , aqueous solution , engineering , biology
Transmembrane lipid exchange is critical to membrane function and pharmaceutical application. The exchange process is not fully understood and it is explored by quartz crystal microbalance with dissipation monitor method in this paper. It is found that the vesicle deformation discrepancy is not significant for the supported-lipid-bilayer-attached vesicles under different thermal and ionic strength conditions. And hence the total intermembrane contact area is determined by the vesicle adsorption amount. The maximum total intermembrane contact area decreases with the increase of temperature and the decrease of ionic strength. The changes of the vesicle adsorption rate and the transmembrane lipid exchange rate induced by temperature and ionic strength are elucidated to understand the observation above. The study helps explain some physiological phenomena and provides some guidelines for drug delivery researches.