Open AccessThreshold value of shear stress in H-22 cells generated sonoporation
Author(s) -
Mo Run-Yang,
Shuyu Lin,
Chenghui Wang
Publication year - 2011
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.60.114306
Subject(s) - sonoporation , sonication , materials science , shear stress , cytoplasm , ultrasound , suspension (topology) , bubble , biophysics , microbubbles , chemistry , composite material , chromatography , mechanics , acoustics , physics , biochemistry , mathematics , homotopy , pure mathematics , biology
Based on a spherically symmetric bubble model, the threshold shear stress for H-22 cells which are irradiated by continuous focused ultrasound is estimated. On the condition that the final concentration of the superparamagnetic iron oxide (SPIO) in the cell suspension is 410 g/mL, the focused ultrasound sonication is at a frequency of 1.37 MHz, the power from amplifer is 2 W, and H-22 cells are exposed to the ultrasound for 60 s, the labeling efficiency is about 45.9%13.5%. Prussian blue staining confirms iron uptake and shows numerous blue-stained iron particles in the cytoplasm, while more than 90% labeled cells remained viable. The results show that the reparable sonoporation arises in plasma membrane and SPIO nanoparticles enter into the H-22 cells. Numerical calculations show that the shear stress acting on the cell is 697 Pa, which is the threshold shear stress for H-22 cell sonoporation.