Open AccessAutomatic procedure for non-coplanar aberration compensation in lensless Fourier transform digital holography
Author(s) -
Huakun Cui,
Dayong Wang,
Yunxin Wang,
Changgeng Liu,
Jie Zhao,
Yan Li
Publication year - 2011
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.60.044201
Subject(s) - holography , autofocus , fourier transform , optics , digital holography , contrast (vision) , computer science , compensation (psychology) , phase (matter) , computer vision , artificial intelligence , physics , focus (optics) , quantum mechanics , psychology , psychoanalysis
When the lensless Fourier transform digital holography is applied to the microscopic phase-contrast imaging on live cells, the motion of cells will lead to a non-coplanary phenomenon between the object recorded and the reference source. This could result in the imaging aberration. An effective and robust autofocus procedure based on the phase distribution is presented in the paper. With the initial measurement of the distance between the reference source and the hologram, the optimal parameters corresponding to the phase-contrast image can be achieved by a single hologram combined with the linearity fitting. Lensless Fourier transform digital holographic system is built and the experiments on the phase-contrast imaging of the live cervical carcinoma cells are performed. Finally, the good experimental results are obtained. Both the theoretical analysis and the experimental investigation verify the feasibility and the validity of the automatic procedure for non-coplanar aberration compensation.