Open AccessNano-resolution imaging of filopodia in HeLa cells
Author(s) -
Lei Liu,
Bin Yu,
Hanben Niu,
Danni Chen
Publication year - 2010
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.59.6948
Subject(s) - resolution (logic) , nano , materials science , full width at half maximum , microscope , optics , fluorescence , image resolution , microscopy , pixel , molecule , fluorescence microscope , optoelectronics , physics , computer science , artificial intelligence , quantum mechanics , composite material
Nano-resolution imaging based on single molecule localization is simulated with Matlab. A sample consisted of several lines of molecules are simulated imaged. The results demonstrate that two lines of molecules separated by 20 nm can be resolved. Effect of pixel size on the localization accuracy is also discussed here. Nano-resolution imaging is realized on an IX71 inverted fluorescent microscope with the resolution (FWHM) of 48 nm. Nano-resolution images of filopodias are reconstructed, in which filopodias with diameter of 75 nm are resolved.