Open AccessMicro-dynamics of Ca2+ signals in single heart cells
Author(s) -
Bai Yong-Qiang,
Tang Ai-Hui,
Shi Qiang Wang,
Zhu Xing
Publication year - 2007
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.56.3607
Subject(s) - microscope , total internal reflection fluorescence microscope , reflection (computer programming) , dynamics (music) , signal (programming language) , field (mathematics) , physics , optics , stochastic resonance , transformation (genetics) , noise (video) , fluorescence , acoustics , computer science , chemistry , artificial intelligence , image (mathematics) , pure mathematics , programming language , biochemistry , gene , mathematics
We used a total internal reflection fluorescence microscope based on the near field optics principle to study the Ca2+ signals in single heart cells of Sprague-Dawley rat. Due to the high signal to noise ratio and high speed of data acquisition of this microscope, the Ca2+ images show complex 2-dimensional wave patterns. The local elementary Ca2+ release units play important role in the formation and transformation of Ca2+ wave. Simulations based on Fire-Diffuse-Fire model show that the Ca2+ wave, composed of Ca2+ release units, may steadily exist in single heart cell. Our results will be helpful for understanding the micro-dynamics of living excitable medium.