Open AccessStudy of interactions between DNA and histone with molecular combing method
Author(s) -
Yuying Liu,
Shixue Dou,
PengYe Wang,
Ping Xie,
Wei-Chi Wang
Publication year - 2005
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.54.622
Subject(s) - histone , combing , dna , base pair , molecule , biophysics , biology , chemistry , materials science , biochemistry , organic chemistry , composite material
The interactions between DNA and histone are studied by using the molecular combing method. With this simple but effective method, λDNA molecules are stretched to a length of 26—28 μm, 16—17 times of their contour length (162 μm). After DNA binding of histones, DNA molecules are condensed and the lengths of the stretched complexes become significantly shorter, with length distribution peak lying between 10—14 μm. The lengths of the stretched DNAhistone complexes are closely related to the histone concentration and the mixing ratio of DNA base pair and dye molecule.