Open AccessFluorescence correlation spectroscopy with saturated excitation
Author(s) -
丁建英,
陈波,
孟凡波,
马辉
Publication year - 2004
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.53.2503
Subject(s) - fluorescence correlation spectroscopy , excitation , spectroscopy , atomic physics , monte carlo method , materials science , fluorescence , diffusion , laser induced fluorescence , fluorescence spectroscopy , laser , fluorescence cross correlation spectroscopy , physics , molecular physics , optics , quantum mechanics , statistics , mathematics , thermodynamics
In this paper,we study the errors caused by an intense excitation beam in fluorescence correlation spectroscopy (FCS). Saturated excitation induced by an intense excitation beam will alter the spatial distribution function of the laser-induced fluorescence; therefore it changes the results of FCS measurements. Using the theory for saturated excitation,we derived a general expression for the effective detection volume as a function of the excitation laser intensity. Quantitative relations between the particle number and diffusion time,both obtained from FCS measurements,and the excitation laser intensity are established and used to remove the systematic error due to saturated excitation. The success of this technique is demonstrated in both Monte-Carlo simulations and experiments.