Open AccessThe application of total internal reflection fluorescence microscopy in single fluorophore molecules axial imaging
Author(s) -
Wang Chen,
Guiying Wang,
Zhizhan Xu
Publication year - 2004
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.53.1325
Subject(s) - total internal reflection fluorescence microscope , fluorophore , microscopy , optics , total internal reflection , fluorescence , fluorescence microscope , physics , reflection (computer programming) , spheres , materials science , computer science , astronomy , programming language
Here we present a method of modeling fluorophore molecules as spheres in evan escent fields mathematically instead of the assumption of tophat function dist r ibution. Based on this model, we discuss that it is possible to calculate relati ve separation distances of the molecules, and their axial positions and diameters with the total internal reflection fluorescence microscopy(TIRFM) due to its uni que intensity pattern in the z direction. In the whole process, we need mere ly to change the incident angle once, that is to get only two frame imaging of TIRFM.