Open AccessTHE IMAGING THEORY OF TWO-PHOTON AND MULTI-PHOTON CONFOCAL SCANNING MICROSCOPY
Author(s) -
Tang Zhi-Lie,
Liang Ruisheng,
Chang Hong-Sen
Publication year - 2000
Publication title -
wuli xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.199
H-Index - 47
ISSN - 1000-3290
DOI - 10.7498/aps.49.1076
Subject(s) - two photon excitation microscopy , confocal , photon , confocal microscopy , optics , microscopy , scanning confocal electron microscopy , materials science , physics , multiphoton fluorescence microscope , fluorescence microscope , fluorescence
The Imaging theory of two-photon and multi-photon confocal scanning microscopy is presented. The three-dimensional point spread function and three-dimensional optical transfer function of two-photon confocal scanning microscopy is derived. By using the three-dimensional point spread function and three-dimensional optical transfer function theory, we have proved that the horizontal and vertical resolutions of two-photon confocal scanning microscopy are higher than those of the single photon confocal scanning microscopy, and the space resolution of multi-photon confocal scanning microscopy is higher than that of two-photon confocal scanning microscopy.