Open AccessAktivitas Antioksidan Ekstrak, Fraksi dan Golongan Senyawa Kimia Daun Premna oblongata Miq.
Author(s) -
Katrin Katrin,
Atika Bendra
Publication year - 2015
Publication title -
pharmaceutical sciences and research
Language(s) - English
Resource type - Journals
eISSN - 2477-0612
pISSN - 2407-2354
DOI - 10.7454/psr.v2i1.3332
Subject(s) - dpph , chemistry , antioxidant , traditional medicine , ethyl acetate , saponin , flavonoid , hexane , column chromatography , chromatography , organic chemistry , medicine , alternative medicine , pathology
Indonesia has many kinds of plants which can be used as natural antioxidant sources. This study was focused on antioxidant activity of Cincau Perdu leaves extract (Premna oblongata Miq.) using 1-Diphenyl-2-picrylhydrazyl (DPPH) methods. P. oblongata leaves was extracted using n-hexane, ethylacetate and methanol. It was found that methanol extract has the highest activity with IC50 value of 20.01 μg/mL. The extract with highest antioxidant activity were fractionated with accelerated column chromatography and were earned 6 combination fractions. The antioxidant activity of each combination fractions were tested by DPPH methods. Fraction-5 showed the highest activity as antioxidant with IC50 of 23.51 μg/mL. The compounds of the active fractions were flavonoid, glikon, saponin and tannin