Open AccessHepatoprotective activity of 95% methanolic extract was investigated against D-Galactosamine  and paracetamol induced hepatotoxicity in wistar albino rats. Silymarin was used as the reference standard at 50mg/kg orally and it showed effect in both the hepatotoxicity models. Rats were divided in 5 groups each group contains 6 animals. The degree of protection was determined by the estimation of biochemical parameter like SGPT, SGOT, ALP, Bilirubin (Direct & Total). In histopathological study was also carried out.The 95% methanolic extract of fruit of Macrotyloma uniflorum (MEMUS) at the dose of (200mg/kg and 400mg/kg) produced a dose dependant  reduction in biochemical parameter like SGPT, SGOT, ALP, Bilirubin (Direct & Total) as well as in morphological parameters in D-Galctosamine and paracetamol induced hepatotoxicity in rats. The histopathological study further supported the hepatoprotective activity of the test extract. Maximum protection was seen at 400mg/kg Macrotyloma uniflorum seed extract. The results of the present investigation indicated that Macrotyloma uniflorum seed has significant hepatoprotactive properties.