Open AccessThe present study aimed to study in-vitro anti-inflammatory and anti-arthritic activity of Eucalyptus globules oil. Anti-inflammatory activity of Eucalyptus globules oil at 100, 250, 500 mcg/ml were studied using human red blood cell membrane (HRBC) stabilization method and anti-arthritic activity of same concentration using bovine serum albumin denaturation(BSA) method. The effect Diclofenec and Aspirin at concentration of 100, 200 mcg/ml were also assessed as standard drug. Results showed significant (53.48%, 63.73% and 70.66% at 100, 250 and 500 mcg/ml of EGO) protection of HRBC and significant inhibition (16.98%, 58.49%, 66.03% at 100, 250 and 500 mcg/ml of EGO) in BSA denaturation and which are comparable to standard drugs diclofenec and aspirin. This result suggests about their potential action as anti-inflammatory and ant-arthritic activity and sets a platform for therapeutic use.