Open AccessMedicinal plants, herbs, spices and herbal remedies are integral components of alternative system of medicine since times immemorial. Stylosanthes fruticosa. is a potential folklore medicinal plant (Fabacea) used for Aurveda and Siddha systems of medicine. In this study Alkaloid, Carbohydrate and Glycoside, Saponin, Protein & Amino acid, Phenolic compounds & Flavonoids and Phytosterols were identified as the major phytochemical constituents in the ethanol fractions of Stylosanthes fruticosa leaf extract. Their structures were elucidated, on the basis of GC-MS data . (2R,3R)-4-methyl-2,3-epoxypentan-1-ol(9.26%),1-Cyclohexyl-2,2-difluoro-4-(1,3-dioxolan-2-yl)-4-iodobutanone(9.26%),9-(Tetrahydropyran-2''-yl)-6-[2'-phenyl-',4',5',6''-tetrapropylphenyl]-9H-purine(9.26%) these different active phytochemicals have been found to possess a wide range of activities. In conclusion Stylosanthes fruticosa contains biologically active compounds that may serve as candidate for the discovery of new drugs in the treatment of antimicrobial activities.