Open AccessIntroduction: Intra ocular pressure is an essential entity in maintaining the structural and functional integrity of eye ball. It varies in regular cycle in the normal eye for 24hour. In the present study, the diurnal variation of Heart rate, intra ocular pressure, systolic, diastolic, pulse and mean arterial pressures from 6AM to 6PM with every 2 hour interval was investigated. Methodology: The present study included 100 normal male healthy subjects from the Gulbarga city, recruited after their informed and written consent. Intra ocular pressure, Heart rate, Blood pressure, Temperature, Urine output and respiratory rate per minute was investigated in all the subjects. Diurnal variation of Intra ocular pressure, Blood pressure, heart rate was measured at every 2hrs from 6AM to 6PM. Result: The diurnal variation of Intra Ocular Pressure was significantly high (p<0.05). The diurnal variation of Heart Rate and Blood Pressure showed variations from 6AM to 6PM within physiological limits. Discussion: The diurnal variation of Intra Ocular Pressure, Heart Rate and Blood Pressure in early morning and at evening might be due to the combined homeostatic mechanism of autonomic nervous system and glucocorticoid release. Conclusion: Hence intra ocular pressure fluctuation more than 30 mmHg during diurnal variation water has diagnostic value towards development of latent Glaucoma.